利用CRISPR/Cas9技术创制大豆高油酸突变系

侯智红,吴艳,程群,董利东,芦思佳,南海洋,甘卓然,刘宝辉

Creation of high oleic acid soybean mutation plants by CRISPR/Cas9

Zhi-Hong HOU,Yan WU,Qun CHENG,Li-Dong DONG,Si-Jia LU,Hai-Yang NAN,Zhuo-Ran GAN,Bao-Hui LIU

图6 GmFAD2-1A突变体鉴定 A: T1代转基因大豆植株的PCR检测; M: DNA marker (DM2000); 1: H2O 空白对照; 2: H3野生型; 3~7: 转基因大豆草铵膦抗性阳性苗。B: GmFAD2-1A突变体与野生型序列比对分析; C: GmFAD2-1A突变体与野生型蛋白序列比对结果。

Fig. 6 Identification of GmFAD2-1A mutant A: PCR detection of T1 transgenic soybean; M: DNA marker (DM2000); 1: H2O blank control; 2: WT H3; 3-7: positive seedling of glufosinate tolerence of transgenic soybean. B: sequence alignment of GmFAD2-1A mutant compared to the WT line; C: sequence alignment of GmFAD2-1A mutant and wild-type protein.