一个CRISPR/Cas9-VQR基因编辑系统的构建

陈凯,孙国梁,宋高原,李爱丽,谢传晓,毛龙,耿帅锋

Establishment of a CRISPR/Cas9-VQR gene editing system

Kai CHEN,Guo-Liang SUN,Gao-Yuan SONG,Ai-Li LI,Chuan-Xiao XIE,Long MAO,Shuai-Feng GENG

表1 OsPDS-gRNAs序列

Table 1 Sequence of OsPDS-gRNAs

靶位点 Target site 序列 Targeted sequence (5′-3′) GC含量 GC content (%) 外显子位置 Location OsPDS-gRNA1 CTTGGAAGGATGAAGATGGAGA 45 3 OsPDS-gRNA2 CCAGGAGAATTCAGCCGGTTTGA 55 6 OsPDS-gRNA3 TCAGGAGAAGCATGGTTCTAAGA 45 8 OsPDS-gRNA4 TCCCGGACTGTGAACCTTGCCGA 60 13