一个CRISPR/Cas9-VQR基因编辑系统的构建

陈凯,孙国梁,宋高原,李爱丽,谢传晓,毛龙,耿帅锋

Establishment of a CRISPR/Cas9-VQR gene editing system

Kai CHEN,Guo-Liang SUN,Gao-Yuan SONG,Ai-Li LI,Chuan-Xiao XIE,Long MAO,Shuai-Feng GENG

表2 本研究所用引物

Table 2 Primers used in this research

引物名称 Primer name 序列 Sequence (5′-3′) T7-gRNA1-FPg TAATACGACTCACTATAGCTTGGAAGGATGAAGATGGGTTTTAGAGCTAGAAATAGC T7-gRNA2-FPg TAATACGACTCACTATAGCCAGGAGAATTCAGCCGGTTGTTTTAGAGCTAGAAATAGC T7-gRNA3-FPg TAATACGACTCACTATAGTCAGGAGAAGCATGGTTCTAGTTTTAGAGCTAGAAATAGC T7-gRNA4-FPg TAATACGACTCACTATAGTCCCGGACTGTGAACCTTGCGTTTTAGAGCTAGAAATAGC gRNA-RP AGCACCGACTCGGTGCCACTT oligo-gRNA1-F TTGCTTGGAAGGATGAAGATGG oligo-gRNA1-R AACCCATCTTCATCCTTCCAAG oligo-gRNA2-F TTGCCAGGAGAATTCAGCCGGTT oligo-gRNA2-R AACAACCGGCTGAATTCTCCTGG oligo-gRNA4-F TTGTCCCGGACTGTGAACCTTGC oligo-gRNA4-R AACGCAAGGTTCACAGTCCGGGA PCR-gRNA1-F TCAGAGTAAAGCAAAGATTC PCR-gRNA1-R TCAGGCTCCTACGAGATA PCR-gRNA2-F GAATTTTCGCTTAGAGGC PCR-gRNA2-R CCAGTTATTTGAGTTCCATC PCR-gRNA4-F TGTGCCTGTATGTAACCA PCR-gRNA4-R GAGCAAACTTCACCTTCT