一个CRISPR/Cas9-VQR基因编辑系统的构建

陈凯,孙国梁,宋高原,李爱丽,谢传晓,毛龙,耿帅锋

Establishment of a CRISPR/Cas9-VQR gene editing system

Kai CHEN,Guo-Liang SUN,Gao-Yuan SONG,Ai-Li LI,Chuan-Xiao XIE,Long MAO,Shuai-Feng GENG

表5 转基因植株突变体数量统计

Table 5 Number of mutants among transgenic plants

名称 Name 阳性植株 Transplant 突变植株 Mutated plant 突变率 Mutation ratio (%) 纯合突变体 Homo plant 杂合突变体 Het plant OsPDS-gRNA1 17/24 12 70.5 4/12 8/12 OsPDS-gRNA2 40/45 11 27.5 5/11 6/11 OsPDS-gRNA4 49/53 20 40.8 7/20 13/20