一个CRISPR/Cas9-VQR基因编辑系统的构建

陈凯,孙国梁,宋高原,李爱丽,谢传晓,毛龙,耿帅锋

Establishment of a CRISPR/Cas9-VQR gene editing system

Kai CHEN,Guo-Liang SUN,Gao-Yuan SONG,Ai-Li LI,Chuan-Xiao XIE,Long MAO,Shuai-Feng GENG

图2 CRISPR/Cas9-VQR载体 ZmUbi: 玉米泛素启动子; NLS: 核定位信号; D1135V: 1135位的天冬氨酸(D)突变为缬氨酸(V); R1335Q: 1335位的精氨酸(R)突变为谷胱氨酸(Q); T1337R: 1337位的苏氨酸(T)突变为精氨酸(R); 3′UTR: 3′非翻译区; Ter: 终止子; gRNA: 指导RNA; OsU6: 水稻U6启动子。

Fig. 2 Construction of CRISPR/Cas9-VQR vector ZmUbi: ubiquitin promoter; NLS: nuclear localization signal; D1135V: substituting the 1135D residue with V; R1335Q: substituting the 1335R residue with Q; T1337R: substituting the 1337 T residue with R; 3′ UTR: 3′ untranslated region; Ter: terminator; gRNA: guide RNA; OsU6: OsU6 promoter.