一个CRISPR/Cas9-VQR基因编辑系统的构建

陈凯,孙国梁,宋高原,李爱丽,谢传晓,毛龙,耿帅锋

Establishment of a CRISPR/Cas9-VQR gene editing system

Kai CHEN,Guo-Liang SUN,Gao-Yuan SONG,Ai-Li LI,Chuan-Xiao XIE,Long MAO,Shuai-Feng GENG

图3 体外酶切检测活性结果 S1和S2: 标准样品1和2; CK1和CK2: 阴性对照1和2; g1: OsPDS-gRNA1; g2: OsPDS-gRNA2; g3: OsPDS-gRNA3; g4: OsPDS-gRNA4。

Fig. 3 Cas9-VQR enzyme digestion activity in vitro S1 and S2: standard sample 1 and 2; CK1 and CK2: negative control 1and 2; g1: OsPDS-gRNA1; g2: OsPDS-gRNA2; g3: OsPDS- gRNA3; g4: OsPDS-gRNA4.