甘蔗脂氧合酶基因ScLOX1的克隆与表达分析

孙婷婷,王文举,娄文月,刘峰,张旭,王玲,陈玉凤,阙友雄,许莉萍,李大妹,苏亚春

Cloning and expression analysis of sugarcane lipoxygenase gene ScLOX1

Ting-Ting SUN,Wen-Ju WANG,Wen-Yue LOU,Feng LIU,Xu ZHANG,Ling WANG,Yu-Feng CHEN,You-Xiong QUE,Li-Ping XU,Da-Mei LI,Ya-Chun SU

表1 本研究所用引物序列及用途

Table 1 Sequence and purpose of the primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5'-3') 用途 Purpose ScLOX1-cDNAF CCATCCATCCACCAACCA RT-PCR ScLOX1-cDNAR ACGGCACAGCACAACATTTA RT-PCR GAPDH-QF CACGGCCACTGGAAGCA qRT-PCR GAPDH-QR TCCTCAGGGTTCCTGATGCC qRT-PCR ScLOX1-QF ATCATTGAGGCTGTTCTT qRT-PCR ScLOX1-QR TCTGCTATTAGTGGATTGT qRT-PCR ScLOX1-Gate-F GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTTCTGGCACGGGGTCGC Transient overexpression vector construction ScLOX1-Gate-R GGGGACCACTTTGTACAAGAAAGCTGGGTCTATGGAGATGCTGTTGGGAA Transient overexpression vector construction M13-F TGTAAAACGACGGCCAGT Transient overexpression vector construction M13-R CAGGAAACAGCTATGACC Transient overexpression vector construction NtHSR201-F CAGCAGTCCTTTGGCGTTGTC qRT-PCR after transient overexpression 引物名称 Primer name 引物序列 Primer sequence (5'-3') 用途 Purpose NtHSR201-R GCTCAGTTTAGCCGCAGTTGTG qRT-PCR after transient overexpression NtHSR203-F TGGCTCAACGATTACGCA qRT-PCR after transient overexpression NtHSR203-R GCACGAAACCTGGATGG qRT-PCR after transient overexpression NtHSR515-F TTGGGCAGAATAGATGGGTA qRT-PCR after transient overexpression NtHSR515-R TTTGGTGAAAGTCTTGGCTC qRT-PCR after transient overexpression NtPR-1a/c-F AACCTTTGACCTGGGACGAC qRT-PCR after transient overexpression NtPR-1a/c-R GCACATCCAACACGAACCGA qRT-PCR after transient overexpression NtPR2-F TGATGCCCTTTTGGATTCTATG qRT-PCR after transient overexpression NtPR2-R AGTTCCTGCCCCGCTTT qRT-PCR after transient overexpression NtPR3-F CAGGAGGGTATTGCTTTGTTAGG qRT-PCR after transient overexpression NtPR3-R CGTGGGAAGATGGCTTGTTGTC qRT-PCR after transient overexpression NtEFE26-F CGGACGCTGGTGGCATAAT qRT-PCR after transient overexpression NtEFE26-R CAACAAGAGCTGGTGCTGGATA qRT-PCR after transient overexpression NtAccdeaminase-F TCTGAGGTTACTGATTTGGATTGG qRT-PCR after transient overexpression NtAccdeaminase-R TGGACATGGTGGATAGTTGCT qRT-PCR after transient overexpression NtEF-1α-F TGCTGCTGTAACAAGATGGATGC qRT-PCR after transient overexpression NtEF-1α-R GAGATGGGGACAAAGGGGATT qRT-PCR after transient overexpression