甘蔗脂氧合酶基因ScLOX1的克隆与表达分析

孙婷婷,王文举,娄文月,刘峰,张旭,王玲,陈玉凤,阙友雄,许莉萍,李大妹,苏亚春

Cloning and expression analysis of sugarcane lipoxygenase gene ScLOX1

Ting-Ting SUN,Wen-Ju WANG,Wen-Yue LOU,Feng LIU,Xu ZHANG,Ling WANG,Yu-Feng CHEN,You-Xiong QUE,Li-Ping XU,Da-Mei LI,Ya-Chun SU

图4 ScLOX1与其他植物LOX蛋白构建的系统发育树 ScLOX1用▲标识。玉米LOXs: ZmLOX1 (AF271894)、ZmLOX3 (AF329371)、ZmLOX6 (DQ335764)、ZmLOX10 (DQ335768)、ZmLOX11 (DQ335769); 水稻LOXs: OsLOX1 (DQ389164)、r9-LOX1 (AB099850); 番茄LOXs: TomLOXA (U09026)、TomLOXB (U09025)、TomLOXC (U37839)、TomLOXD (U37840)、TomLOXE (AY008278)、TomLOXF (FJ617476); 拟南芥LOXs: AtLOX1 (NM_104376)、AtLOX2 (AY062611)、AtLOX3 (AJ249794)、AtLOX4 (NM_105911); 烟草LOXs: NaLOX1 (X84040)、NaLOX2 (AY254348)、NaLOX3 (AY254349); 马铃薯LOXs: StLOX1 (X95513)、StLOX2 (X96405)、StLOX3 (X96406); 大豆LOXs: GmLOX9 (EU003576)、GmLOX10 (EU003577); 花生LOX: PnLOX2 (DQ068249); 豌豆LOX: LOXN2 (AJ749702); 桃LOXs: PpLOX1 (EU883638)、PpLOX2 (FJ029110)、PpLOX3 (FJ032015)、PpLOX4 (EF568783); 葡萄LOXs: VvLOXA (FJ858255)、VvLOXC (FJ858256)、VvLOXO (FJ858257); 橄榄LOXs: OeLOX (EU678670)、Oep1LOX2 (EU513352)、Oep2LOX2 (EU513353); 辣椒LOXs: CaLOX1 (FJ377708)、CaLOX2 (JQ219046); 杨树LOXs: PdLOX1 (DQ131178)、PdLOX2 (DQ131179); 杏LOXs: LOX1:Pd:1 (AJ404331)、LOX1:Pd:2 (AJ418043); 菜豆LOX: PvLOX6 (EF196866); 甘蓝LOX: BoLOX (EF123056); 鬼箭锦鸡儿LOX: CjLOX (EF530043); 茶树LOX: CasLOX1 (EU195885); 榛子LOX: CaLOX (AJ417975)。

Fig. 4 Phylogenetic tree estimated by the ScLOX1 and those proteins from other plants ScLOX1 is marked with a black triangle. Zea mays LOXs: ZmLOX1 (AF271894), ZmLOX3 (AF329371), ZmLOX6 (DQ335764), ZmLOX10 (DQ335768), ZmLOX11 (DQ335769); Oryza sativa LOXs: OsLOX1 (DQ389164), r9-LOX1 (AB099850); Lycopersicum esculentum LOXs: TomLOXA (U09026), TomLOXB (U09025), TomLOXC (U37839), TomLOXD (U37840), TomLOXE (AY008278), TomLOXF (FJ617476); Arabidopsis thaliana LOXs: AtLOX1 (NM_104376), AtLOX2 (AY062611), AtLOX3 (AJ249794), AtLOX4 (NM_105911); Nicotiana tabacum LOXs: NaLOX1 (X84040), NaLOX2 (AY254348), NaLOX3 (AY254349); Solanum tuberosum LOXs: StLOX1 (X95513), StLOX2 (X96405), StLOX3 (X96406); Glycine max LOXs: GmLOX9 (EU003576), GmLOX10 (EU003577); Arachis hypogaea LOX: PnLOX2 (DQ068249); Pisum sativum LOX: LOXN2 (AJ749702); Prunus persica LOXs: PpLOX1 (EU883638), PpLOX2 (FJ029110), PpLOX3 (FJ032015), PpLOX4 (EF568783); Vitis vinifera LOXs: VvLOXA (FJ858255), VvLOXC (FJ858256), VvLOXO (FJ858257); Olea europaea LOXs: OeLOX (EU678670), Oep1LOX2 (EU513352), Oep2LOX2 (EU513353); Capsicum annuum LOXs: CaLOX1 (FJ377708), CaLOX2 (JQ219046); Populus deltoids LOXs: PdLOX1 (DQ131178), PdLOX2 (DQ131179); Prunus dulcis LOXs: LOX1:Pd:1 (AJ404331), LOX1:Pd:2 (AJ418043); Phaseolus vulgaris LOX: PvLOX6 (EF196866); Brassica oleracea LOX: BoLOX (EF123056); Caragana jubata LOX: CjLOX (EF530043); Camellia sinensis LOX: CasLOX1 (EU195885); Corylus avellana LOX: CaLOX (AJ417975).