甘蔗脂氧合酶基因ScLOX1的克隆与表达分析

孙婷婷,王文举,娄文月,刘峰,张旭,王玲,陈玉凤,阙友雄,许莉萍,李大妹,苏亚春

Cloning and expression analysis of sugarcane lipoxygenase gene ScLOX1

Ting-Ting SUN,Wen-Ju WANG,Wen-Yue LOU,Feng LIU,Xu ZHANG,Ling WANG,Yu-Feng CHEN,You-Xiong QUE,Li-Ping XU,Da-Mei LI,Ya-Chun SU

图5 甘蔗ScLOX1与其他植物LOX氨基酸序列比对 文本框表示底物结合结构域(Domain I和II)和C末端保守区域。箭头表示与铁结合有关的保守氨基酸残基。加号为TH(V)/R(K), 是一个对确定加氧位置特异性至关重要的基序。OsLOX1: 水稻(DQ389164); ZmLOX1: 玉米(AF271894); HvLOXC: 大麦(L37358); SbLOX4: 高粱(XP_002466613); SiLOX4: 谷子(XP_ 004982082)。

Fig. 5 Amino acids sequence aligenment of ScLOX1 and other plant LOXs The substrate-binding domain (Domains I and II) and C-terminus conserved regions are shown by text boxes. An arrow indicates a conserved amino acid residue related to iron binding. The plus sign indicates TH(V)/R(K), a motif proposed to be essential for determining oxygen-adding positional specificity. OsLOX1: Oryza sativa (DQ389164); ZmLOX1: Zea mays (AF271894); HvLOXC: Hordeum vulgare (L37358); SbLOX4: Sorghum bicolor (XP_ 002466613); SiLOX4: Setaria italica (XP_004982082).