异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

图1 陆地棉GhSAMDC1和其他物种同源蛋白进化树分析及转基因鉴定 A: 陆地棉GhSAMDC1和其他物种同源蛋白进化树分析; B: GhSDAMC1表达载体; C: 转GhSAMDC1基因拟南芥DNA鉴定及表达分析, M、1、2、3、4、5分别为marker、转基因株系1-4、转基因株系1-12、转基因株系1-14、阳性对照、阴性对照, 目的基因片段大小为1035 bp; D: 转GhSAMDC1基因拟南芥表达分析。

Fig. 1 Phylogenetic analysis of homologous proteins of upland cotton GhSAMDC1 and other species and identification of transgenic Arabidopsis thaliana A: phylogenetic analysis of homologous proteins of upland cotton GhSAMDC1 and other species; B: expression vector of GhSDAMC1; C: DNA identification of transgenic Arabidopsis thaliana (GhSAMDC1), M, 1, 2, 3, 4, and 5 were marker, transgenic line 1-4, transgenic line 1-12, transgenic line 1-14, positive control, and negative control, respectively, the size of the target gene fragment was 1035 bp; D: expression analysis of GhSAMDC1 in Arabidopsis thaliana.