异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

图3 过量表达GhSAMDC1基因对拟南芥抗盐能力的影响 A: 种子分布示意图; B~C: 正常和盐胁迫下培养15 d后野生型及转基因拟南芥表型; D~E: 正常培养15 d后野生型和转基因拟南芥叶片数及鲜重; F~H: 100 mmol L-1 NaCl处理15 d后野生型和转基因拟南芥成活率、鲜重及叶片数。野生型和转基因拟南芥在正常培养和100 mmol L-1 NaCl处理15 d后统计鲜重、叶片数和成活率。数据采用Duncan法进行差异显著性检验(* P < 0.05, ** P < 0.01), 结果用平均值±标准误表示, 以上实验均使用3次生物学重复, 每次实验3次技术重复。

Fig. 3 Effect of overexpression of GhSAMDC1 on salt tolerance in Arabidopsis thaliana A: schematic diagram of seed distribution; B-C: phenotypes of wild type and transgenic Arabidopsis thaliana cultivated under normal and salt stress for 15 days; D-E: fresh weight and leaf number of wild type and transgenic Arabidopsis thaliana after normal culture for 15 days; F-H: survival rate, fresh weight and leaf number of wild type and transgenic Arabidopsis thaliana after 100 mmol L-1 NaCl treatment for 15 days. Duncan method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard), the above experiments were repeated three times biologically and three times technically in each experiment.