异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

附图1 300 mmol L-1 NaCl处理下棉花GhSAMDC1基因表达分析 0、1、3、6、12、24、48、72分别代表棉花幼苗(YZ-1)用300 mmol L-1 NaCl处理0、1、3、6、12、24、48和72 h。培养30 d的棉花幼苗用300 mmol L-1 NaCl处理, 分别在0、1、3、6、12、24、48和72 h取样, 检测不同处理时间段GhSAMDC1基因相对表达量。以上实验均使用3次生物学重复, 每次实验3次技术重复。

Supplementary Fig. 1 Expression analysis of GhSAMDC1 in cotton under 300 mmol L-1 NaCl treatment 0, 1, 3, 6, 12, 24, 48, and72 represent cotton seedlings (YZ-1) after normal culture for 30 days treated with 300 mmol L-1 NaCl for 0, 1, 3, 6, 12, 24, 48, and 72 hours, respectively. Samples were taken at 0, 1, 3, 6, 12, 24, 48, and 72 hours of treatments to detect the relative expression of GhSAMDC1 in different treatment periods. The above experiments were repeated three times biologically and three times technically in each experiment.