异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

图5 盐胁迫下过量表达GhSAMDC1基因对拟南芥内源多胺含量的影响 A~D: 正常和盐胁迫下野生型及转基因株系内源的多胺总量、Put、Spd和Spm含量对比。选取正常培养和100 mmol L-1 NaCl处理15 d的拟南芥叶片, 利用高效液相色谱法检测内源多胺含量。数据采用Duncan法进行差异显著性检验(* P < 0.05, ** P < 0.01), 结果用平均值±标准差表示, 以上实验均使用3次生物学重复, 每次实验3次技术重复。

Fig. 5 Effects of overexpression of GhSAMDC1 on endogenous polyamine content in Arabidopsis thaliana under salt stress A-D: comparison of total endogenous polyamines, Put, Spd, and Spm contents between wild type and transgenic lines under normal and salt stress conditions. The contents of endogenous polyamines in Arabidopsis thaliana leaves were determined by high performance liquid chromatography (HPLC) after normal culture and treatment with 100 mmol L-1 NaCl for 15 days. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment.