异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

图7 盐胁迫下过量表达GhSAMDC1基因对拟南芥叶片H2O2、MDA及离子渗透率影响 A: 盐胁迫下野生型和转基因株系DAB染色; B~D: 盐胁迫下野生型和转基因株系H2O2、MDA含量及离子渗透率; 选取100 mmol L-1 NaCl处理15 d的拟南芥叶片, 分别进行DAB染色, H2O2、MDA含量以及离子渗透率的检测, 数据采用Duncan法进行差异显著性检验(* P < 0.05, ** P < 0.01), 结果用平均值±标准差表示, 以上实验均使用3次生物学重复, 每次实验3次技术重复。

Fig. 7 Effects of overexpression of GhSAMDC1 on contents of H2O2, MDA, and ion permeability in Arabidopsis thaliana leaves under salt stress A: DAB staining of wild type and transgenic lines under salt stress; B-D: contents of H2O2, MDA and ion permeability of wild type and transgenic lines under salt stress. The leaves of Arabidopsis thaliana were stained with DAB, and the contents of H2O2, MDA and ion permeability were measured after treated with 100 mmol L-1 NaCl for 15 days. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologically and three times technically in each experiment.