异源表达棉花S-腺苷甲硫氨酸脱羧酶(GhSAMDC1)基因提高了拟南芥抗盐能力

田文刚,朱雪峰,宋雯,程文翰,薛飞,朱华国

Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana

Wen-Gang TIAN,Xue-Feng ZHU,Wen SONG,Wen-Han CHENG,Fei XUE,Hua-Guo ZHU

图8 盐胁迫下过量表达GhSAMDC1基因对拟南芥抗氧化酶活力和表达的影响 A~C: 盐胁迫下野生型和转基因株系CAT、SOD、POD活力; D~F: 盐胁迫下野生型和转基因株系CAT、SOD、POD表达分析。选取100 mmol L-1 NaCl处理15 d的拟南芥叶片, 分别检测CAT、SOD和POD的活力和相对表达量。数据采用Duncan法进行差异显著性检验(* P < 0.05, ** P < 0.01), 结果用平均值±标准差表示, 以上实验均使用3次生物学重复, 每次实验3次技术重复。

Fig. 8 Effects of overexpression of GhSAMDC1 on activity and expression of antioxidant enzymes in Arabidopsis thaliana under salt stress A-C: enzyme activity of CAT, SOD, and POD in wild type and transgenic lines under salt stress; D-F: analysis of CAT, SOD, and POD expression in wild type and transgenic lines under salt stress. The leaves of Arabidopsis thaliana were selected to detect the activity and relative expression of CAT, SOD, and POD after treated with 100 mmol L-1 NaCl for 15 days, respectively. Duncan’s method was used to test the difference significance (* P < 0.05, ** P < 0.01), the results were represented by mean (±standard deviation), the above experiments were repeated three times biologic ally and three times technically in each experiment.