花生AhPEPC1基因抑制表达的转基因后代转录组分析

潘丽娟,陈娜,陈明娜,王通,王冕,陈静,杨珍,万勇善,禹山林,迟晓元,刘风珍

Transcriptome analysis of the peanut transgenic offspring with depressing AhPEPC1 gene

Li-Juan PAN,Na CHEN,Ming-Na CHEN,Tong WANG,Mian WANG,Jing CHEN,Zhen YANG,Yong-Shan WAN,Shan-Lin YU,Xiao-Yuan CHI,Feng-Zhen LIU

表3 RNA-seq与RT-PCR验证倍数差别

Table 3 Differental fold-change between RNA-seq and RT-PCR

基因编号 Gene ID 基因描述 Gene description RNA-seq变化倍数 RNA-seq fold change 调控模式 Regulation RT-PCR变化倍数 RT-PCR fold change Aradu.2N2A3 Protein IQ-DOMAIN 14 53.7 Up 2.2 Aradu.32P5Q Transketolase, chloroplastic 164.3 Down 2.1 Aradu.38MP3 — 61.8 Up 1.7 Aradu.3UA04 Pollen-specific protein SF21 11.2 Down 16.8 Aradu.8XK1K DNA polymerase alpha catalytic subunit 2.3 Up 1.1 Aradu.91JGK Probable leucine-rich repeat receptor-like serine/threonine-protein kinase 10.5 Down 1.6 Aradu.94LL9 F-box/FBD/LRR-repeat protein 4.7 Up 1.9 Aradu.AAS86 ABC transporter G family member 36 2.4 Up 3.0 Aradu.CZ64T Protein FAR1-RELATED SEQUENCE 12 5.2 Down 2.4 Aradu.FE0Z7 Cystathionine beta-lyase, chloroplastic 137.4 Down 1.4 Aradu.G395P Copper-transporting ATPase PAA1, chloroplastic 30.4 Down 1.5 Aradu.JE7IW Pollen-specific protein SF21 25.1 Down 2.9 Aradu.M0JX8 Cystathionine beta-lyase, chloroplastic 201.1 Down 2.0 Aradu.PU7RJ MADS-box transcription factor 6 2.9 Down 3.4 Aradu.Y0LYZ Myb-like protein 2.0 Up 2.4