小麦类受体蛋白激酶基因TaPK3A的克隆与抗纹枯病功能初步分析

苏强,荣玮,张增艳

Cloning and functional analysis of wheat receptor-like kinase gene TaPK3A

Qiang SU,Wei RONG,Zeng-Yan ZHANG

图3 小麦品系CI12633中TaPK3A基因的表达分析 A: TaPK3A基因在抗纹枯病小麦CI12633中的表达分析, 横坐标表示禾谷丝核菌侵染小麦CI12633后0 h、2 d、4 d、7 d、10 d、14 d和21 d时的样品。B: CI12633小麦不同组织中TaPK3A受禾谷丝核菌的诱导表达分析。C: TaPK3A基因响应 SA、JA、ABA和 ET的表达分析, 横坐标表示不同植物激素处理小麦CI12633后0 h、0.5 h、1 h、6 h和24 h时的样品。t检验分析TaPK3A表达量的差异显著性, *P < 0.05; **P < 0.01。

Fig. 3 Expression analysis of TaPK3A in wheat line CI12633 A: expression analysis of TaPK3A in resistant wheat line CI12633. 0 h, 2 d, 4 d, 7 d, 10 d, 14 d, and 21 d stand for the samples of wheat line CI12633 after inoculation of R. cerealis. B: expression analysis of TaPK3A induced by R. cerealis in the organs of wheat line CI12633. C: expression analysis of TaPK3A after SA, JA, ABA, and ET treatment. 0 h, 0.5 h, 1 h, 6 h, and 24 h stand for the samples of wheat line CI12633 after different phytohormones treatments, t-test was used to statistically analyze the significant difference of TaPK3A expression. * P < 0.05; ** P < 0.01.