小麦类受体蛋白激酶基因TaPK3A的克隆与抗纹枯病功能初步分析

苏强,荣玮,张增艳

Cloning and functional analysis of wheat receptor-like kinase gene TaPK3A

Qiang SU,Wei RONG,Zeng-Yan ZHANG

图4 BSMV CP、TaPK3A基因的检测及小麦花叶和纹枯病表型 A: 半定量PCR检测CP的转录表达, 扩增26个循环; B: 对接种BSMV: GFP和BSMV: TaPK3A 10 d后的植株RT-qPCR检测TaPK3A的表达, **P < 0.01 (t检验); C: 接种BSMV后10 d, 小麦叶片呈现出BSMV病毒斑; D: 接种禾谷丝核菌后30 d小麦茎秆上纹枯病斑, IT: 病级

Fig. 4 Expression analysis of CP and TaPK3A and the symptoms of BSMV and sharp eyespot in BSMV-VIGS wheat A: transcriptional expression of CP by semi-quantitative PCR, and the amplification was 26 cycles. B: RT-qPCR analysis of the relative transcript levels of TaPK3A in the wheat plants infected by BSMV: GFP or BSMV: TaPK3A at 10 dpi. **P < 0.01 (t-test). C: BSMV virus spots on wheat leaves 10 days later after inoculation. D: sharp eyespot symptoms on wheat stems 30 days after inoculation with R. cerealis. IT: infection type.