不同收获指数甘蓝型油菜β-淀粉酶活性及其基因家族成员的表达分析
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靳舒荣,王艳玫,常悦,王月华,李加纳,倪郁
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Activity and gene family expression of β-amylase in Brassica napus differing in harvest index
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Shu-Rong JIN,Yan-Mei WANG,Yue CHANG,Yue-Hua WANG,Jia-Na LI,Yu NI
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表1 qPCR相关引物
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Table 1 Sequences of the primers used in qPCR
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名称 Name | 正向引物 Forward primer (5°-3°) | 反向引物 Reverse primer (5°-3°) | 扩增片段大小 Size (bp) | BAM1 | ATTGGAGCTTTCCAGTGCTATGACA | GCCAGTTATTGTAGTGACCAGCATCG | 124 | BAM3 | GATGTGACTCTGTGCCTGTCCTCA | CTCCACAAGGTCCCATTCCCA | 141 | BAM4 | ATTCTTCTGGTTGCCCTATCATTAAA | GACAGAAACGCTCCACAATCCC | 126 | BAM5 | CAGATCATAGGAGAAGCCAACAAGA | GCGTGGCTGTGGTGGTTGTA | 101 | Actin7 | GTGACAATGGAACTGGAATGGTGA | GTGCCTAGGACGACCAACAATACTC | 92 |
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