小麦抗逆相关基因TaSAP1的5′非翻译区内含子功能分析

常建忠,董春林,张正,乔麟轶,杨睿,蒋丹,张彦琴,杨丽莉,吴佳洁,景蕊莲

Function analysis of 5′ untranslated region introns in drought-resistance gene TaSAP1

Jian-Zhong CHANG,Chun-Lin DONG,Zheng ZHANG,Lin-Yi QIAO,Rui YANG,Dan JIANG,Yan-Qin ZHANG,Li-Li YANG,Jia-Jie WU,Rui-Lian JING

表1 TaSAP1启动子主要顺式作用元件及预测的功能

Table 1 Main cis-acting elements and predicted functions in the TaSAP1 promoter sequence

元件名称 Name 序列 Sequence 功能 Function 位置 Location (bp) ABRE GACACGTGGC/TACGTG ABA响应 cis-acting element involved in abscisic acid responsivenes -937 to -926, -510 to -504 ARE AAACCA 低氧响应 cis-acting regulatory element essential for anaerobic induction -1256 to -1249 Box-W1 GGTCAA 真菌诱导响应 Fungal elicitor responsive element -2062 to -2055 CGTCA-motif CGTCA 茉莉酸甲酯响应 cis-acting regulatory element involved in MeJA-responsiveness -2107 to -2101 CCAAT-box CCGTTG MYB转录因子结合元件 MYB binding site -1176 to -1169, -658 to -651 GC-motif CGGGGGC 低氧特异增强元件 Enhancer-like element involved in anoxic specific inducibility -1534 to -1526 LTR CCGAAA 低温响应 cis-acting element involved in low-temperature responsiveness -1233 to -1226, -1207 to -2199 MBS TGACCGA MYB转录因子结合元件 MYB binding site -1448 to -1440, -39 to -32 Skn-1 motif GTCAT 胚乳特异表达元件 cis-acting regulatory element required for endosperm expression -1323 to -1317, -1108 to -1102 TGACG-motif TGACG 茉莉酸甲酯响应 cis-acting regulatory element involved in MeJA-responsiveness -1914 to -1908 WUN-motif TCATTGCGAA 伤害响应 Wound-responsive element -2103 to -2092