水稻OsUBA基因的表达及其在促进种子萌发和开花中的功能

张双双,王立伟,姚楠,郭光艳,夏玉凤,秘彩莉

Expression of OsUBA and its function in promoting seed germination and flowering

Shuang-Shuang ZHANG,Li-Wei WANG,Nan YAO,Guang-Yan GUO,Yu-Feng XIA,Cai-Li BI

图9 35S:OsUBA载体的PCR及双酶切鉴定 A: pCAMBIA1300-OsUBA的菌液PCR; M: 5 kb marker; 1: 阳性克隆; B: pCAMBIA1300-OsUBA质粒的Xba I/Kpn I双酶切鉴定; M: 5 kb marker; 1: 阳性克隆质粒(未酶切); 2: 阳性克隆质粒的Xba I/Kpn I双酶切。

Fig. 9 Identification of 35S:OsUBA vector by PCR and double digestion A: amplification of positive clone harboring pCAMBIA1300- OsUBA; M: 5 kb marker; 1: positive clone; B: identification of pCAMBIA1300-OsUBA positive clone by Xba I/Kpn I digestion; M: 5 kb marker; 1: plasmid of pCAMBIA1300-OsUBA (undigested); 2: Xba I/Kpn I digestion of pCAMBIA1300-OsUBA plasmid.