大麦蛋白质二硫键异构酶基因家族的鉴定与表达分析

时丽洁,蒋枞璁,王方梅,杨平,冯宗云

Genome-wide characterization and transcriptional analysis of the protein disulfide isomerase-like genes in barley (Hordeum vulgare)

Li-Jie SHI,Cong-Cong JIANG,Fang-Mei WANG,Ping YANG,Zong-Yun FENG

表3 qPCR引物及扩增片段

Table 3 Primers used for qPCR and length of amplified fragments

引物名称 Primer name 引物序列 Sequence (5'-3') 退火温度 Tm (°C) 扩增片段大小 Size of amplicon (bp) 备注 Remarks HvUBC-F AAGCAGCCAGAATGTACAGCGAGAAC 63 152 内参基因 Reference gene HvUBC-R GGTACAGACCAGCAAAGCCAGAAATG BaMMV-F GGCACTTTGTGTTATGCAATGG 63 138 检测BaMMV Check the presence of BaMMV BaMMV-R TGCCACCCTCAGGTAGGATTAG HvPDIL1_1-F CGTTGCTTGGTTGAAGGATT 60 199 检测基因表达 Check the expression of HvPDIL1-1 HvPDIL1_1-R GATGGGTGCTAGCTTCTTGC HvPDIL1_2-F CAGACCCGACCAACCATAAG 60 194 检测基因表达 Check the expression of HvPDIL1-2 HvPDIL1_2-R ATACTCGAAGGCACGGTCAG 引物名称 Primer name 引物序列 Sequence (5'-3') 退火温度 Tm (°C) 扩增片段大小 Size of amplicon (bp) 备注 Remarks HvPDIL1_3-F GTGTGGCCATTGTCAGTCAC 60 200 检测基因表达 Check the expression of HvPDIL1-3 HvPDIL1_3-R CCCCCTCGAAAGTTATAGGC HvPDIL1_4-F AGTTGGCCAAGCATTTCAGT 60 198 检测基因表达 Check the expression of HvPDIL1-4 HvPDIL1_4-R CTGCAGCTTCTCCTTGATGA HvPDIL2_1-F CGGGACTTGGATGACTTTGT 60 200 检测基因表达 Check the expression of HvPDIL2-1 HvPDIL2_1-R AGGACCACTGAGCTTTGCAG HvPDIL2_2-F CTAGCGCAGATGTGATGGAA 60 198 检测基因表达 Check the expression of HvPDIL2-2 HvPDIL2_2-R GCTTCTCCAGATCAGCTTGC HvPDIL5_1-F CGAAGAGACCTTCTCCGACA 60 200 检测基因表达 Check the expression of HvPDIL5-1 HvPDIL5_1-R GAATGTCCACCTTTGAGCAGA HvPDIL5_2-F CCCCGAGTTAGATGAAGCTG 60 199 检测基因表达 Check the expression of HvPDIL5-2 HvPDIL5_2-R AAGATTGCGGACAAGCAAGT HvPDIL5_3-F GAAACTTCCATGCGGTTGAT 60 199 检测基因表达 Check the expression of HvPDIL5-3 HvPDIL5_3-R CCAAACTCTCCAAACCCTCA HvPDIL5_4-F CCTGAAATGGATGGCAGAAT 60 200 检测基因表达 Check the expression of HvPDIL5-4 HvPDIL5_4-R TCCATAGCCGCGACTAAACT