2个抗虫棉的外源Bt基因分子鉴定及其染色体定位

周向阳,赵亮,狄佳春,陈旭升

Molecular identification and chromosomal mapping of exogenous Bt gene in two insect-resistant cotton varieties

Xiang-Yang ZHOU,Liang ZHAO,Jia-Chun DI,Xu-Sheng CHEN

表2 33B×胜利1号杂交后代Bt基因的分离情况

Table 2 Bt gene separation in hybrid offspring of 33B crossing V-1

世代 Generation 亲本及组合 Parent and combination 含Bt基因单株数 Number of individuals with Bt gene 不含Bt基因单株数 Number of individuals without Bt gene χ2值 χ2 value P1 V-1 0 84 — P2 33B 90 0 — F1 P2×P1 76 0 — F2 F1代自交 Self-crossing F1 148 37 2.47