大豆出苗期耐盐性鉴定方法建立及耐盐种质筛选

刘谢香,常汝镇,关荣霞,邱丽娟

Establishment of screening method for salt tolerant soybean at emergence stage and screening of tolerant germplasm

Xie-Xiang LIU,Ru-Zhen CHANG,Rong-Xia GUAN,Li-Juan QIU

表1 盐胁迫后大豆出苗期和苗期的盐害症状

Table 1 Symptoms of soybean at emergence and seedling stages under salt stress

出苗期 Emergence stage 苗期 Seedling stage 类别 Category 表型特征 Symptom 级别 Grade 表型特征 Symptom I 植株凋亡, 子叶干枯(类别数值为1) Plant dead, cotyledons were dry (category value is 1) 1 健康的绿叶, 没有观察到损伤 Healthy green leaves, no damage observed II 植株生长受到严重抑制, 子叶未展开(类别数值为2) Plant growth was severely inhibited, cotyledon was not unfolded (category value is 2) 2 轻度坏死, 真叶轻微发黄 Slight chlorosis, light yellowish color observed in true leaves III 植株生长受到抑制, 具有生长点, 但真叶未展开(类别数值为3) Plant growth was inhibited with shoot apical meristem, but true leaves were not unfolded (category value is 3) 3 中度坏死, 三出复叶发黄 Moderate chlorosis, chlorosis observed in trifoliate leaves IV 植株生长基本正常, 真叶未完全展开(类别数值为4) Plant growth was basically normal, true leaves were not fully expanded (category value is 4) 4 严重坏死, 超过75%的叶面发黄 Severe chlorosis, more than 75% of the leaf area showed chlorosis V 植株生长正常, 真叶完全展开(类别数值为5) Plant growth was normal, true leaves were fully expanded (category value is 5) 5 凋亡, 植物完全枯萎 Dead, plants were completely withered