过表达OsMPK17激酶蛋白质增强了水稻的耐旱性

马金姣,兰金苹,张彤,陈悦,郭亚璐,刘玉晴,燕高伟,魏健,窦世娟,杨明,李莉云,刘国振

Overexpression of OsMPK17 protein enhances drought tolerance of rice

Jin-Jiao MA,Jin-Ping LAN,Tong ZHANG,Yue CHEN,Ya-Lu GUO,Yu-Qing LIU,Gao-Wei YAN,Jian WEI,Shi-Juan DOU,Ming YANG,Li-Yun LI,Guo-Zhen LIU

图2 水稻OsMPK17蛋白质的表达特征分析 A: 干旱胁迫: 发芽后生长5 d的水稻幼苗在20% PEG-6000中水培, 分别于胁迫处理的0、1 h、2 h、4 h、8 h、12 h、1 d 、2 d和3 d取地上部样品, 提取总蛋白质后进行免疫印迹(Western blot, WB)分析。CK: 对照; Drought: PEG-6000胁迫处理。HSP: 以HSP82抗体检测的信号作为上样内参。B: 激素处理: 剪下水稻幼苗叶片在培养皿中培养, 分别添加100 μmol L-1 ABA或100 μmol L-1 MeJA进行激素处理, 取材时间点为0、6 h、12 h、1 d、2 d、3 d、4 d、5 d和6 d, 以水培为CK, 提取叶片样品总蛋白质进行WB分析。HSP: 以HSP82抗体检测的信号作为上样内参。

Fig. 2 Expression profiling of OsMPK17 protein in rice by western blot analysis A: Drought stress treatment: rice seedlings grown for 5 days were treated by 20% PEG-6000. Leaf samples were collected at 0, 1 h, 2 h, 4 h, 8 h, 12 h, 1 d, 2 d, and 3 d respectively; WB analysis were carried out for isolated total proteins. HSP: Loading control for WB analysis using HSP82 antibody. B: Treatment with hormones: leaves of rice were cultured in petri dish, 100 μmol L-1 ABA or 100 μmol L-1 MeJA was supplemented as hormone treatments. Samples were collected at 0, 6 h, 12 h, 1 d, 2 d, 3 d, 4 d, 5 d, and 6 d time points, respectively. Total proteins were isolated and analyzed by WB. HSP: Loading control for WB analysis using HSP82 antibody.