过表达OsMPK17激酶蛋白质增强了水稻的耐旱性

马金姣,兰金苹,张彤,陈悦,郭亚璐,刘玉晴,燕高伟,魏健,窦世娟,杨明,李莉云,刘国振

Overexpression of OsMPK17 protein enhances drought tolerance of rice

Jin-Jiao MA,Jin-Ping LAN,Tong ZHANG,Yue CHEN,Ya-Lu GUO,Yu-Qing LIU,Gao-Wei YAN,Jian WEI,Shi-Juan DOU,Ming YANG,Li-Yun LI,Guo-Zhen LIU

图4 过表达OsMPK17蛋白质转基因水稻筛选与鉴定 上部: PCR结果; 中部: WB检测转基因水稻中OsMPK17蛋白质; 下部: HSP检测的信号为上样参照。WT: 野生型; A202、A204、A210和A 212为不同的转基因株系; 1、2、3、4、5、6、7、8和9为同一株系内不同的单株; PCR: PCR产物; MPK17-OX: 过表达的OsMPK17蛋白质; MPK17-Native: 水稻中原来的OsMPK17蛋白质。

Fig. 4 Identification of transgenic rice plants with overexpression OsMPK17 protein Upper panel: PCR product; Middle panel: WB detection of OsMPK17 protein in transgenic rice plants; Lower panel: HSP signal was used as loading control; WT: wildtype rice plants; A202, A204, A210, and A212 are independent transgenic lines; 1, 2, 3, 4, 5, 6, 7, 8, and 9 are independent plants among the same transgenic lines; PCR: PCR products; MPK17-OX: Over expressed OsMPK17 protein; MPK17-Native: the original form of OsMPK17 protein in rice.