利用酵母双杂交系统筛选大豆结瘤因子受体NFR1α的互作蛋白

柯丹霞,彭昆鹏

Screening of NFR1α-interactive proteins in soybean using yeast two hybrid system

Dan-Xia KE,Kun-Peng PENG

图1 诱饵质粒pGBKT7-GmNFR1α-pk的构建及自激活检测 (A) M: trans 2K Plus II DNA marker; 1: GmNFR1α-pk目的片段扩增; 2: pGBKT7-GmNFR1α-pk诱饵质粒双酶切; 3: pGBKT7空载体双酶切。(B)阳性对照(pGBKT7-53和pGADT7-T)。(C)阴性对照(pGBKT7-lam和pGADT7-T)。(D) pGBKT7-GmNFR1α-pk与pGADT7空载体。

Fig. 1 Construction and auto-activation test of the bait plasmid pGBKT7-GmNFR1α-pk (A) M: trans 2K Plus II DNA marker; 1: isolation of GmNFR1α-pk cDNA; 2: pGBKT7-GmNFR1α-pk digested by EcoR I and Sal I; 3: pGBKT7 digested by EcoR I and Sal I. (B) positive control (pGBKT7-53 and pGADT7-T). (C) negative control (pGBKT7-lam and pGADT7-T). (D) pGBKT7-GmNFR1α-pk and pGADT7.