利用酵母双杂交系统筛选大豆结瘤因子受体NFR1α的互作蛋白

柯丹霞,彭昆鹏

Screening of NFR1α-interactive proteins in soybean using yeast two hybrid system

Dan-Xia KE,Kun-Peng PENG

图3 GmNFR1α与GmLbc2相互作用的验证 (A)酵母双杂交系统验证GmNFR1α-pk与GmLbc2的相互作用。1: 阳性对照(pGBKT7-53和pGADT7-T); 2: 阴性对照(pGBKT7-lam和pGADT7-T); 3: pGBKT7-GmNFR1α-pk与pGADT7-GmLbc2。(B)双分子荧光互补技术验证GmNFR1α全长与GmLbc2的相互作用。

Fig. 3 Interaction between GmNFR1α and GmLbc2 (A) yeast two-hybrid analysis of interaction between GmNFR1α-pk and GmLbc2. 1: positive control (pGBKT7-53 and pGADT7-T); 2: negative control (pGBKT7-lam and pGADT7-T); 3: pGBKT7-GmNFR1α-pk and pGADT7-GmLbc2. (B) BiFC analysis of interaction between GmNFR1α and GmLbc2.