利用酵母双杂交系统筛选大豆结瘤因子受体NFR1α的互作蛋白

柯丹霞,彭昆鹏

Screening of NFR1α-interactive proteins in soybean using yeast two hybrid system

Dan-Xia KE,Kun-Peng PENG

图6 过表达GmLbc2对百脉根阳性毛根共生结瘤的影响 (A)空载体对照复合体植株的结瘤表型。(B)超表达GmLbc2 (GmLbc2-OX)复合体植株的结瘤表型; 接种根瘤菌30 d 后照相, Bars = 5 mm。(C)平均每个植株的结瘤数目, **表示P<0.01。(D) qRT-PCR检测GmLbc2、NIN、Enod40-1和Enod40-2 的表达水平。

Fig. 6 Effect of GmLbc2 overexpression on nodulation in L. japonicus (A) phenotype of hairy roots expressing p1301U (CK). (B) phenotype of hairy roots overexpressing GmLbc2 (GmLbc2-OX); photographs were taken at 30 d after inoculation, Bars = 5 mm. (C) mean number of nodules per plant with standard deviation (SD) of L. japonicus expressing empty vector pU1301 (CK) of GmLbc2-OX at 30 days after inoculation with M. loti, **P<0.01. (D) transcript levels of GmLbc2, NIN, Enod40-1, and Enod40-2 in CK and GmLbc2-OX hairy roots detected by qRT-PCR.