陆地棉钾转运体基因GhHAK5启动子的克隆与功能分析

晁毛妮,胡海燕,王润豪,陈煜,付丽娜,刘庆庆,王清连

Cloning and functional analysis of promoter of potassium transporter gene GhHAK5 in upland cotton (Gossypium hirsutum L.)

Mao-Ni CHAO,Hai-Yan HU,Run-Hao WANG,Yu CHEN,Li-Na FU,Qing-Qing LIU,Qing-Lian WANG

表1 GhHAK5启动子区包含的顺式作用元件

Table 1 cis-acting elements in promoter of GhHAK5

元件类型 Element type 名称 Name 拷贝数 Copy number 基序 Motif sequence 功能 Function 基础元件 Basal element 5UTR Py-rich stretch 1 TTTCTTCTCT 高转录水平顺式作用元件 cis-acting element conferring high transcription levels TATA-box 61 TATA/ATATAT/TTTTA 转录起始位点-30核心启动子元件 Core promoter element around -30 of transcription start CAAT-box 18 CAATT/CAAT/CCAAT 启动子和增强子区的一般顺式作用元件 Common cis-acting element in promoter and enhancer regions 光 Light Box I 1 TTTCAAA 光响应元件 Light responsive element I-box 1 ATGATATGA 部分光响应元件 Part of a light responsive element G-Box 1 CACGTT 光响应顺式作用调控元件 cis-acting regulatory element involved in light responsiveness AT1-motif 1 ATTAATTTTACA 部分光响应模块 Part of a light responsive module Box II 1 GTGGATATTATAT 部分光响应元件 Part of a light responsive element 生物钟 Circadian Circadian 1 CAANNNNATC 昼夜节律顺式作用调控元件 cis-acting regulatory element involved in circadian control 植物激素 Phytohormone CGTCA-motif 1 CGTCA 茉莉酸响应顺式作用元件 cis-acting regulatory element involved in the MeJA-responsiveness TCA-element 1 GAGAAGAATA 水杨酸响应顺式作用元件 cis-acting element involved in salicylic acid responsiveness TGA-element 1 AACGAC 生长素响应元件 Auxin-responsive element ERE 1 ATTTCAAA 乙烯响应元件 Ethylene-responsive element 逆境胁迫 Stress Box-W1 1 TTGACC 真菌诱导子响应元件 Fungal elicitor responsive element HSE 4 AAAAAATTTC 热胁迫响应顺式作用元件 cis-acting element involved in heat stress responsiveness 其他 Other Skn-1_motif 4 GTCAT 胚乳表达相关顺式调控元件 cis-acting regulatory element required for endosperm expression AT-rich element 1 ATAGAAATCAA AT-rich DNA结合蛋白的结合位点(ATBP-1) Binding site of AT-rich DNA binding protein Root-specific motif 5 ATAAAAT 根特异性表达响应元件 Root-specific responsive element