陆地棉钾转运体基因GhHAK5启动子的克隆与功能分析

晁毛妮,胡海燕,王润豪,陈煜,付丽娜,刘庆庆,王清连

Cloning and functional analysis of promoter of potassium transporter gene GhHAK5 in upland cotton (Gossypium hirsutum L.)

Mao-Ni CHAO,Hai-Yan HU,Run-Hao WANG,Yu CHEN,Li-Na FU,Qing-Qing LIU,Qing-Lian WANG

图2 GhHAK5启动子的序列分析 核苷酸的+1位置指的是起始密码子ATG中的A的位置, ATG用红色表示。部分预测的顺式作用元件在图中用灰色阴影表示。1个ARF转录因子结合位点(TGTCNN)在图中用红色加下画线表示。5个根特异性表达元件(ATAAAAT)在图中用红色加粗表示。

Fig. 2 Sequence analysis of GhHAK5 promoter The nucleotide at position +1 is the ATG start codon, the ATG is indicated in red. Parts of the putative cis-regulatory elements are noted under the sequences in shadow. The one ARF binding site (TGTCNN) is underlined in red, and the five root-specific motifs are bold in red.