陆地棉钾转运体基因GhHAK5启动子的克隆与功能分析

晁毛妮,胡海燕,王润豪,陈煜,付丽娜,刘庆庆,王清连

Cloning and functional analysis of promoter of potassium transporter gene GhHAK5 in upland cotton (Gossypium hirsutum L.)

Mao-Ni CHAO,Hai-Yan HU,Run-Hao WANG,Yu CHEN,Li-Na FU,Qing-Qing LIU,Qing-Lian WANG

图4 T1代转基因拟南芥植株的PCR检测 M: marker; 1: 野生型拟南芥(阴性对照); 2: 表达载体pCAMBIA1381Z-pGhHAK5质粒(阳性对照); 3~8转基因拟南芥。

Fig. 4 PCR analysis of T1 generation transgenic Arabidopsis plants M: marker; 1: wild Arabidopsis thaliana (negative control); 2: expression vector pCAMBIA1381Z-pGhHAK5 plasmid (positive control); 3-8: transgenic Arabidopsis thaliana.