小麦类胡萝卜素合成途径关键基因Lcye功能分析
翟胜男, 郭军, 刘成, 李豪圣, 宋健民, 刘爱峰, 曹新有, 程敦公, 李法计, 何中虎, 夏先春, 刘建军

Functional analysis of Lcye gene involved in the carotenoid synthesis in common wheat
Sheng-Nan ZHAI, Jun GUO, Cheng LIU, Hao-Sheng LI, Jian-Min SONG, Ai-Feng LIU, Xin-You CAO, Dun-Gong CHENG, Fa-Ji LI, Zhong-Hu HE, Xian-Chun XIA, Jian-Jun LIU
表2 Lcye基因的qRT-PCR引物信息
Table 2 Primers of Lcye gene for qRT-PCR analysis
基因Gene 名称Name 序列Sequence (5'-3')
Lcye-all Lcye-all-F2 TGACCACYGAATATCCAGTTGC
Lcye-all-R6 AGTTTTCTTTGAGGAAACATGC
Lcye-A1 Lcye-A1-F7 GTTGCTGAGAAGATGCAACGAT
Lcye-A1-R7 CAAAGTATCTTGCGGTCCCTTT
Lcye-B1 Lcye-B1-F3 ATCTCCAGATGGACATCGAGTG
Lcye-B1-R3 TCCAACCTCATACTCTAGAAGT
Lcye-D1 Lcye-D1-F3 TTGGCCCTGATCTTCCATTC
Lcye-D1-R1 ATATACTACTCGATGTCCATCA
β-actin Actin-F CTGATCGCATGAGCAAAGAG
Actin-R CCACCGATCCAGACACTGTA