小麦类胡萝卜素合成途径关键基因Lcye功能分析

翟胜男, 郭军, 刘成, 李豪圣, 宋健民, 刘爱峰, 曹新有, 程敦公, 李法计, 何中虎, 夏先春, 刘建军

Functional analysis of Lcye gene involved in the carotenoid synthesis in common wheat

Sheng-Nan ZHAI, Jun GUO, Cheng LIU, Hao-Sheng LI, Jian-Min SONG, Ai-Feng LIU, Xin-You CAO, Dun-Gong CHENG, Fa-Ji LI, Zhong-Hu HE, Xian-Chun XIA, Jian-Jun LIU

表3 利用TILLING技术筛选获得Lcye突变体信息

Table 3 Summary of mutations in Lcye identified by TILLING

基因 Gene 突变体编号 Number of M2 plant 外显子/内含子 Exon/intron 核苷酸改变 Nucleotide change 密码子改变 Codon change 氨基酸改变 Amino acid change 基因型 Zygosity Lcye-A1 M091034 Intron C1184T Hom M091686 Intron C1243T Hom M091772 Intron C1418T Hom M092043 Intron C1478T Hom M092852 Intron C3222T Hom M090431 Intron C858T Het M090631 Intron T1461C Het M090897 Intron G1068A Hom M091996 Intron G1575A Hom M090147 Intron G3073A Hom M091648 Exon G3284A GGA→GAA G392E Hom M090201 Exon G3306A TTA→TTG L399= Hom Lcye-B1 M091626 Intron G2406A Hom Lcye-D1 M092404 Intron C2014T Het M091884 Intron C2017T Het M090945 Exon C2086T TAC→TAT Y214= Hom M092089 Exon C2087T CTC→TTC L215F Hom M091328 Exon C2121T CCT→CTT P226L Het M090815 Exon C2202T TCT→TTT S253F Het M092230 Exon G2195A GCA→ACA A251T Hom M091075 Exon G2262A GGT→GAT G273D Hom