用全基因组关联分析筛选甘蓝型油菜叶片叶绿素含量候选基因

荐红举, 霍强, 高玉敏, 李阳阳, 谢玲, 魏丽娟, 刘列钊, 卢坤, 李加纳

Selection of candidate genes for chlorophyll content in leaves of Brassica napus using genome-wide association analysis

Hong-Ju JIAN, Qiang HUO, Yu-Min GAO, Yang-Yang LI, Ling XIE, Li-Juan WEI, Lie-Zhao LIU, Kun LU, Jia-Na LI

表1 候选基因引物

Table 1 List of primer sequences for candidate genes

基因Gene 引物序列Primer sequence (5°-3°) BnaA04g00600D F: AAATGTCGTTGAGGAATTACGC; R: TGAATGATATACGTCAGCACCA BnaC09g54390D F: AAGGAGCTAACACAATGACTGA; R: GGTATCATCACAGGTAACCCAT BnaC03g18820D F: AATCATGTTTCCATTAGCCACG; R: CATTACCACCGTTGAAAACCAA BnaC06g24160D F: AATGTTGCGCATTATCTTCCTC; R: TGTTTGTAGTGTTTCCAGGAGT BnaC07g39280D F: AGAAAACGATGCTTATGCTGAC; R: CATAGCTGCAGAGATATCGCTA BnaA04g00500D F: AGCTCATATACATTGTGGAGCA; R: TGCTGCTAGATTGGTCTAAGAG BnaA02g24670D F: CTAATCGAATCAAGCCCTGTTG; R: GGTGAAAACGCAAAGAAATTGG BnaC02g01240D F: CTCAAGGAGCTATTGGAATTGC; R: TATCCAGCAATAGGTGTACGAC BnaC03g20950D F: CTTTATGGTGTTGGCCAAGTAC; R: AACTTTACCACGCCGTATTTTC BnaC05g38600D F: GACTCTCTGAAGATACTGAGGC; R: ACTCTTGATCACCGTATACGTC BnaA10g02050D F: GAGCTGGAACAATAACTCCTCT; R: CACAGTTTCTTTGCCTTCTCTG BnaC05g10770D F: GAGTGTTCCTGATTGTATGCAC; R: AGATGTGCCAATCCAAAAGAAC BnaC04g01850D F: GATAGGAACGGTGATGGTTTTG; R: TTCCGTCACGAAATTAAAACCC BnaC02g25030D F: GTAAATGTGCCTGATGAGGTTG; R: TGAACAATCTCTTCGTCAAGGA BnaA06g06000D F: GTCGCTGCTTAAACAGTTACAA; R: GAAACCCAACAAAGAGTGAACA BnaA05g01720D F: GTTCACAACGTCAATGATCACA; R: GTGGCCCAAGAATCAAAGATTT BnaC08g27000D F: GTTTCTGAACGAACAGGTTGAA; R: ATTTAAGCAGCAGCAGAAGTTC BnaC09g05970D F: TTACTAGCTTGGCTCACACTAC; R: CGTTCTCATCAACAACACTCAG BnaC09g05970D F: TTACTAGCTTGGCTCACACTAC; R: CGTTCTCATCAACAACACTCAG BnaA01g20460D F: TTATCAAGCGTGCGAAGTAAAG; R: CGCGCTGATCTTAACCTAGTTA BnaC08g27310D F: TTTGCTACCTGTTGAAAACGAC; R: TTATTCAACTGTGACAAAGCCG