茶树己糖激酶基因CsHXK2的启动子克隆及表达特性分析
李娜娜, 刘莹, 张豪杰, 王璐, 郝心愿, 张伟富, 王玉春, 熊飞, 杨亚军, 王新超

Promoter cloning and expression analysis of the hexokinase gene CsHXK2 in tea plant (Camellia sinensis)
Na-Na LI, Ying LIU, Hao-Jie ZHANG, Lu WANG, Xin-Yuan HAO, Wei-Fu ZHANG, Yu-Chun WANG, Fei XIONG, Ya-Jun YANG, Xin-Chao WANG
表2 茶树CsHXK2基因启动子的主要顺式作用元件
Table 2 Key cis-acting elements in the CsHXK2 promoter of the tea plant
位点名称
Site name
序列
Sequence
功能
Function
-10PEHVPSBD TATTCT 叶绿体基因表达; 生理节律; 光调节。
Chloroplast gene expression; circadian rhythms; light regulation.
ARFAT TGTCTC ARF (生长素响应因子)结合位点; 生长素信号。
ARF (auxin response factor) binding site; auxin signaling.
ARR1AT NGATT ARR1结合元件; 细胞分裂素响应元件。
ARR1-binding element; cytokinin-responsive element.
BOXLCOREDCPAL ACCWWCC MYB结合位点; 苯丙氨酸解氨酶基因; 激发处理; UV-B辐射; 稀释效应。
MYB binding site; phenylalanine ammonia-lyase gene; elicitor treatment; UV-B irradiation; dilution effect.
CACTFTPPCA1 YACT 叶肉特异性基因表达。
Mesophyll-specific gene expression.
CANBNNAPA CNAACAC 胚和胚乳特异转录元件; 种子; 贮藏蛋白。
Element required for embryo and endosperm-specific transcription; seed; storage protein.
CARGATCONSENSUS CCWWWWWWGG 开花时间。
Flowering time.
CCAATBOX1 CCAAT 热激元件。
Heat shock element.
CURECORECR GTAC 铜响应元件; 氧响应元件。
Copper-responsive element; oxygen-response element.
DOFCOREZM AAAG Dof蛋白核心结合位点; 碳代谢调控。
Core site required for binding of Dof proteins; regulating the carbon metabolism.
ELRECOREPCRP1 TTGACC 激发响应元件; WRKY结合位点; 水杨酸/病菌/创伤诱导信号。
Elicitor responsive element; WRKY protein binding site; salicylic acid/pathogen/wound- induced signaling.
ERELEE4 AWTTCAAA 乙烯响应元件; 衰老。
Ethylene responsive element; senescence.
GARE1OSREP1 TAACAGA 赤霉素响应元件; 种子。
Gibberellin-responsive element; seed.
GATABOX GATA 光响应元件。
Light-responsive element.
GT1CONSENSUS GRWAAW 光响应元件。
Light-responsive element.
LTRE1HVBLT49 CCGAAA 低温响应元件。
Low-temperature-responsive element.
MYB1AT WAACCA MYB识别位点; 干旱/ABA响应元件。
MYB recognition site; dehydration/ABA-responsive element.
MYBCORE CNGTTR MYB结合位点; 干旱/水响应元件; 黄酮类生物合成。
MYB binding site; dehydration/water-responsive element; flavonoid biosynthesis.
MYBGAHV TAACAAA 赤霉素响应元件; MYB结合位点; α-淀粉酶。
Gibberellin response element; MYB binding site; α-amylase.
MYCCONSENSUSAT CANNTG MYC识别位点; 低温/干旱/ABA响应元件。
MYC recognition site; cold/dehydration/ABA-responsive element.
NTBBF1ARROLB ACTTTA 组织特异性表达与生长素诱导。
Tissue-specific expression and auxin induction.
POLLEN1LELAT52 AGAAA 花粉特异性表达。
Pollen specific expression.
RAV1AAT CAACA RAV1结合位点; 莲座叶和根。
RAV1 binding site; rosette leaves and roots.
RHERPATEXPA7 KCACGW 根毛特异性元件; 根; 毛。
Root hair-specific cis-element; root; hair.
位点名称
Site name
序列
Sequence
功能
Function
SREATMSD TTATCC 糖抑制元件; 腋芽生长。
Sugar-repressive element; axillary bud outgrowth.
SURE1STPAT21 AATAGAAAA 蔗糖响应元件。
Sucrose responsive element.
TAAAGSTKST1 TAAAG 保卫细胞特异性基因表达。
Guard cell-specific gene expression.
WBBOXPCWRKY1 TTTGACY WRKY结合位点。
WRKY binding site.
WBOXHVISO1 TGACT 糖响应元件; 糖信号; WRKY结合位点。
Sugar-responsive element; sugar signaling; WRKY binding site.