油菜抗咪唑啉酮类除草剂基因标记的开发与应用

胡茂龙, 程丽, 郭月, 龙卫华, 高建芹, 浦惠明, 张洁夫, 陈松

Development and application of the marker for imidazolinone-resistant gene in Brassica napus

Mao-Long HU, Li CHENG, Yue GUO, Wei-Hua LONG, Jian-Qin GAO, Hui-Ming PU, Jie-Fu ZHANG, Song CHEN

表2 KASP标记KALS1R19681913B在小群体中的分型结果与抗性表型

Table 2 KASP KBA1R19681913B genotyping of the small population and the corresponding phenotypes

材料 Material 标记分型 Genotyping 测序结果 Sequecing 抗性特性 Resistance 材料来源 Source M9-2 A A R M9后代株系 M9 progeny line M9-14 A A R M9后代株系 M9 progeny line N341 G G S MICMS恢复系 MICMS restorer line 3075R G G S MICMS恢复系 MICMS restorer line F1-2 G/A G/A R F1(N341×M9-2) F1 hybrid F1-3 G/A G/A R F1(3075R×M9-14) F1 hybrid M318-1 G G S F2(N341×M9-2)单株 F2 plant M318-2 A A R F2(N341×M9-2)单株 F2 plant M318-3 G G S F2(N341×M9-2)单株 F2 plant M318-4 A A R F2(N341×M9-2)单株 F2 plant M318-5 G/A G/A R F2(N341×M9-2)单株 F2 plant M318-6 A A R F2(N341×M9-2)单株 F2 plant M318-7 G/A G/A R F2(N341×M9-2)单株 F2 plant M318-8 A A R F2(N341×M9-2)单株 F2 plant M318-9 G/A G/A R F2(N341×M9-2)单株 F2 plant M318-10 A A R F2(N341×M9-2)单株 F2 plant M318-11 A A R F2(N341×M9-2)单株 F2 plant M318-12 G/A G/A R F2(N341×M9-2)单株 F2 plant