油菜抗咪唑啉酮类除草剂基因标记的开发与应用

胡茂龙, 程丽, 郭月, 龙卫华, 高建芹, 浦惠明, 张洁夫, 陈松

Development and application of the marker for imidazolinone-resistant gene in Brassica napus

Mao-Long HU, Li CHENG, Yue GUO, Wei-Hua LONG, Jian-Qin GAO, Hui-Ming PU, Jie-Fu ZHANG, Song CHEN

表4 标记KBA1R19681913B在鉴定杂交种子纯度的应用

Table 4 Application of the marker KBA1R19681913B in the purity identification of hybrid rapeseed

授粉方式 Different pollination conditions 除草剂处理 IMI treatment 鉴定株数 Numbers of plants 标记鉴定纯度 Seed purity identification by the markers (%) 田间鉴定纯度 Seed purity identification in the field (%) 杂种纯度提高 Increased seed purity after IMI treatment (%) 人工杂交授粉 Artificial hybridization 喷药Treated 117 100 100 — 不喷药Untreated 90 100 100 — 网罩隔离授粉 Hybridization in tents 喷药Treated 130 97 97 13 不喷药Untreated 194 84 84 — 天然授粉 Natural pollination 喷药Treated 165 96 96 16 不喷药Untreated 155 80 80 —