甘薯抗旱相关基因IbNAC72的克隆与功能分析

张欢, 杨乃科, 商丽丽, 高晓茹, 刘庆昌, 翟红, 高少培, 何绍贞

Cloning and functional analysis of a drought tolerance-related gene IbNAC72 in sweet potato

Huan ZHANG, Nai-Ke YANG, Li-Li SHANG, Xiao-Ru GAO, Qing-Chang LIU, Hong ZHAI, Shao-Pei GAO, Shao-Zhen HE

表1 IbNAC72基因克隆和功能分析所用引物

Table 1 Primers used for IbNAC72 gene cloning and functional analysis

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Function NAC-1 CGGAAAGTTGGGATCAAGAA 3′-RACE扩增 NAC-2 CAGGAAGACCGGAAGTTCAA 3′-RACE amplification NAC-3 TTGAACTTCCGGTCTTCCTG 5′-RACE扩增 NAC-4 ATTCGCCCTGCTAGGAAGAT 5′-RACE amplification NAC-F ATGGGTGTGAAAGATATGGACC ORF、DNA序列扩增 NAC-R TTACTGCCTGAACGCTAAGCTAC ORF and DNA sequence amplification NAC-QF TCTTCAACAAGACGACAAGTCAAG RT-qPCR检测 NAC-QR AGGAAAACTCAATGCCATTG RT-qPCR detection Actin-F AGCAGCATGAAGATTAAGGTTGTAGCAC 甘薯内参基因检测 Actin-R TGGAAAATTAGAAGCACTTCCTGTGAAC Sweet potato internal control NAC-OE-F (Xba I) GCTCTAGAATGGGTGTGAAAGATATGGACC 过表达载体构建 NAC-OE-R (Sac I) CGAGCTCTTACTGCCTGAACGCTAAGCTAC Construction of overexpression vector