甘薯抗旱相关基因IbNAC72的克隆与功能分析

张欢, 杨乃科, 商丽丽, 高晓茹, 刘庆昌, 翟红, 高少培, 何绍贞

Cloning and functional analysis of a drought tolerance-related gene IbNAC72 in sweet potato

Huan ZHANG, Nai-Ke YANG, Li-Li SHANG, Xiao-Ru GAO, Qing-Chang LIU, Hong ZHAI, Shao-Pei GAO, Shao-Zhen HE

图4 IbNAC72转基因烟草植株的获得及鉴定 A~D: IbNAC72基因转化烟草的转化、筛选和再生过程; E~I: 转基因烟草植株叶片、茎段和根系的GUS染色呈阳性, WT呈阴性; H: IbNAC72转基因烟草植株的PCR分析; I: 转基因烟草植株IbNAC72基因的表达分析。M: DL2000 marker; W: 水作为阴性对照; P: 质粒pCAMBIA3301-121-IbNAC72作为阳性对照; C: WT作为阴性对照; WT: 野生型烟草植株; T1~T3: 过表达IbNAC72烟草株系。数据表示为平均值 ± SD (n = 3)。** 表示用Student’s t-test在0.01水平差异显著。

Fig. 4 Production and identification of IbNAC72 transgenic tobacco plants A-D: transformation, screening and regeneration of IbNAC72 transgenic tobacco plants; E-I: the leaves, stems, and roots of the overexpression plants show positive reactions in the GUS assay, whereas no GUS expression is detected in WT; H: PCR analysis of GUS-positive plants; I: transcript levels of IbNAC72 in transgenic and WT plants. M: BL2000 DNA markers; W: water as a negative control; P: pCAMBIA3301-121-IbNAC72 as a positive control; C: WT as a negative control; WT: wild-type tobacco plants; T1-T3: IbNAC72-overexpressing tobacco lines. The error bars indicate ± SD (n = 3). ** indicates significant difference at the 0.01 probability level based on Student’s t-test.