甘薯抗旱相关基因IbNAC72的克隆与功能分析

张欢, 杨乃科, 商丽丽, 高晓茹, 刘庆昌, 翟红, 高少培, 何绍贞

Cloning and functional analysis of a drought tolerance-related gene IbNAC72 in sweet potato

Huan ZHANG, Nai-Ke YANG, Li-Li SHANG, Xiao-Ru GAO, Qing-Chang LIU, Hong ZHAI, Shao-Pei GAO, Shao-Zhen HE

图5 IbNAC72基因的过表达提高了转基因烟草植株的抗旱性 A: 干旱胁迫4周后, 转基因烟草和野生型烟草植株的生长状态比较; B: 干旱胁迫4周后, 转基因烟草和野生型烟草植株株高的比较分析; C: 干旱胁迫4周后, 转基因烟草和野生型烟草植株根系的生长状态比较; D: 干旱胁迫4周后, 转基因烟草和野生型烟草植株根系鲜重的比较分析。数据表示为平均值 ± SD (n = 3)。** 表示用Student’s t-test在0.01水平差异显著。缩写同图4。

Fig. 5 Overexpression of IbNAC72 enhances drought tolerance of transgenic tobacco plants A: phenotypes of transgenic plants and WT grown for four weeks under drought stress; B: plant height of transgenic plants and WT grown for four weeks under drought stress; C: root phenotype of transgenic plants and WT grown for four weeks under drought stress; D: fresh weight of the root of transgenic plants and WT grown for four weeks under drought stress. The error bars indicate ± SD (n = 3). ** indicates significant difference at the 0.01 probability level based on Student’s t-test. Abbreviations are the same as those given in Fig. 4.