大豆分枝数相关分子标记开发及qBN-18位点精细定位

吴海涛, 张勇, 苏伯鸿, Lamlom F Sobhi, 邱丽娟

Development of molecular markers and fine mapping of qBN-18 locus related to branch number in soybean (Glycine max L.)

Hai-Tao WU, Yong ZHANG, Bo-Hong SU, F Sobhi Lamlom, Li-Juan QIU

表1 精细定位所用分子标记

Table 1 Molecular markers information for fine mapping

引物名称 Primer name 物理位置 Physical position (bp) 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') BARCSOYSSR_18_1777 54,744,147-54,744,204 CGTTCTTGCATTAAAGGTGGA AACTTCATTGAATTGACGGTGA BARCSOYSSR_18_1791 54,975,880-54,975,949 TGACCAGTCAATTGTTCATTCTTT TTTACTCAACCATCTCCGCA BARCSOYSSR_18_1797 55,076,726-55,076,764 AAGCAAAGAGAACCAAAGCG AAAACACGAAAAGGAAGGCA BARCSOYSSR_18_1803 55,244,106-55,244,173 TTTCGCACTCAATGTCCGTA GTTTCCAAACCACATGGACC BARCSOYSSR_18_1825 55,701,309-55,701,352 GAATCCACCATCACCAAACC CAATGGCAACCCAGTAAGGT BARCSOYSSR_18_1827 55,714,562-55,714,637 GCCCCACTCGATGAAATAAA GCTTTGGCAGAAATTCAAGG BARCSOYSSR_18_1831 55,763,781-55,763,814 TGTTTTTGTTAAATCTTTTGTTTGG TGTGTATGTTTGTGTGTGCACTT BARCSOYSSR_18_1832 55,775,899-55,775,922 AAAAGCTGAGAGCACAAGGC CGTGCTTTTTCAGTCCCATT BARCSOYSSR_18_1834 55,877,756-55,877,781 TTGAAGAGGAGAGAAGAATGGTG GGATGTGATTGTTAGAAAAGAAGAA BARCSOYSSR_18_1841 55,990,705-55,990,750 TGCACGAGGCCATTACATAG TGAAGCGCATATGACTCAACTT BARCSOYSSR_18_1847 56,075,087-56,075,116 TGGTAGGAGTACTCTGAAGTCATTTTT AGCGCTCAAATGAGATTCCT BARCSOYSSR_18_1852 56,172,124-56,172,157 TGTGTGCGTAAGGGAGATCA CTACCAACCTCCGCATGTCT BARCSOYSSR_18_1856 56,223,172-56,223,217 TGGCCATATGCCTAGCTGAT ATGGTGAGCAAACGTCATTG BARCSOYSSR_18_1875 56,797,313-56,797,366 TGAAAAAGAACGTGTTCAAAATG CGAGTTTCATTCTCGGAAGC