大豆分枝数相关分子标记开发及qBN-18位点精细定位

吴海涛, 张勇, 苏伯鸿, Lamlom F Sobhi, 邱丽娟

Development of molecular markers and fine mapping of qBN-18 locus related to branch number in soybean (Glycine max L.)

Hai-Tao WU, Yong ZHANG, Bo-Hong SU, F Sobhi Lamlom, Li-Juan QIU

表2 两个InDel标记的位置、引物序列及预期扩增片段长度

Table 2 Positions of two InDel markers, the primers and the expected length of the amplified fragment

引物名称 Primer name 物理位置 Physical position (bp) 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') 片段长度Product size (bp) 垦农24 Kennong 24 垦丰19 Kenfeng 19 BR69 55,882,229-55,882,378 AGTTGACAGGAACTAAAGTC GATAATTCAAGTAAATAGCGA 156 15 BR77 55,946,063-55,946,342 TCTCTTTGTGTATGTCTTCTCC TTGTTGCATCCAAATGAGAG 268 280