白菜型油菜粒色主效基因BrTT1的调控机制分析

王艳花, 荐红举, 邱晓, 李加纳

Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L.

Yan-Hua WANG, Hong-Jiu JIAN, Xiao QIU, Jia-Na LI

附表2 用于载体构建的引物序列

Supplementary Table 2 Primers sequences for vector construction

引物名称 Primer name 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) Yb-F/-R CCGGAATTCATGTTTTCATCACTCTCAAACCACT CGCGGATCCTTAAAAGTGCGTTTCAGAGACAGA T2-F/-R CCGGAATTCATGATGAGAAAGAGAGAAAGTAGTA CGCGGATCCCTAACAATTAAAGTCCCAGAGACAA T3-F/-R CCGGAATTCATGATTCTGGAGGAGAAAGATGCAT CCCATCGATTTAGCTATCTGAACGTTTTGGATCG T7-F/-R CCGGAATTCATGGTGATGGGTACACCGTCTTCGT CGCGGATCCTCAAACAGGAACGCTGTGCAAGACC T8-F/-R CCGGAATTCATGGATGAATTAAGTATTATACCGT CGCGGATCCCTAGAGTTTATTATTATATATGATT T18-F/-R CCGGAATTCATGGGTAGCAAGATGCTGTTCAGTT CGCGGATCCTTAGCTTGCTTCTGGCAATGTGATC TG1-F/-R CCGGAATTCATGGACAACTCAGCTCCGGACTCCT CGCGGATCCTCAAACTCTAAGGAGCTGCATTTTG TG2-F/-R CCGGAATTCATGGAGGTGAAAGAGAGTAAGAGAG CGCGGATCCTCAAATGGCTTGATTAGAATGTTGT T10-F/-R CCGGAATTCATGACAAGCACTGATCAGACCACCG CGCGGATCCTTAGGCCCATCGGTTTGTCTTAAAG T12-F/-R CCGGAATTCATGGGGAGGAAGACGTGGTTCGACG CGCGGATCCTCAGAAGAGATAATCAAGTATCTGA T5-F/-R CCGGAATTCATGAGACATTCTTGCTGTTATAAAC CGCGGATCCCTAAAGGGACTGACCAAAAGAGACA F/R-TT1 ATGTTTTCATCACTCTCAAACCACT TTAAAAGTGCGTTTCAGAGACAGA gCb-F/-R CACCATGTTTTCATCACTCTCAAACCACT AAAGTGCGTTTCAGAGACAGA F35S3ND/CB-R GGAAGTTCATTTCATTTGGAGAG AAAGTGCGTTTCAGAGACAGA FBTT1I GGATCCGACGTCGAGATCTATAAACATTCCC