白菜型油菜粒色主效基因BrTT1的调控机制分析

王艳花, 荐红举, 邱晓, 李加纳

Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L.

Yan-Hua WANG, Hong-Jiu JIAN, Xiao QIU, Jia-Na LI

图5 酵母双杂筛选BrTT1可能的互作蛋白 酵母细胞涂布于-Leu/-Trp/-Ade/-His的培养基, pGADT7- T/pGBKT7-53作为阳性对照, pGADT7-T/pGBKT7-laminC作为阴性对照。X-α-gal的染色深度表示互作强弱。

Fig. 5 Screening for interactors of BrTT1 by a yeast two-hybrid system Yeast cells were co-transformed and plated on -Leu/-Trp/-Ade/-His plates, with pGADT7-T/pGBKT7-53 as the positive control and pGADT7-T/pGBKT7-laminC as the negative control. X-α-gal activity was determined by colony-lift filter assay; the color intensity of the colonies depicts the qualitative binding strength of the interaction.