白菜型油菜粒色主效基因BrTT1的调控机制分析

王艳花, 荐红举, 邱晓, 李加纳

Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L.

Yan-Hua WANG, Hong-Jiu JIAN, Xiao QIU, Jia-Na LI

图6 过量和干扰表达BrTT1基因 A: 转基因种子中BrTT1的差异表达; O1~O4: 过量表达转基因株系; Ri1~Ri4: 干扰表达转基因株系; B: 非转基因黑籽材料; DAF: 开花后天数。B: 左、中、右分别为过量表达、干扰表达BrTT1基因、非转基因褐籽对照成熟种子表型。标尺 = 5000 μm。

Fig. 6 Expression of BrTT1 in RNAi and over-expression lines A: differential expression analysis of BrTT1 in seeds of the transgenic lines; O1-O4: over-expression lines; Ri1-Ri4: RNAi expression lines; B: non-transgenic lines as the control; DAF: days after flowering. B: left, middle and right represent seed color phenotype in transgenic lines of over-expression, RNAi expression, and the non-transgenic black seed lines, respectively. Bar = 5000 μm.