白菜型油菜粒色主效基因BrTT1的调控机制分析

王艳花, 荐红举, 邱晓, 李加纳

Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L.

Yan-Hua WANG, Hong-Jiu JIAN, Xiao QIU, Jia-Na LI

附图1 PCR扩增检测RNAi BrTT1得到的阳性转基因植株 检测引物为F35S3ND/FBTT1I (附表2), 检测片段1.0 kb。+表示阳性对照。

Supplementary Fig. 1 PCR amplification of a transgene fragment in transgenic plants with BrTT1 down-regulated by RNAi using genomic DNA isolated from the seedlings as templates The primer of F35S3ND/FBTT1I ( Supplementary Table 2) was used for the amplification of a fragment size at about 1.0 kb. + is a positive control.