甘蓝型春油菜早花位点加密及位点聚合创建优异早花资源

柳海东, 潘云龙, 杜德志

Locus encryption for early flowering and QTL polymerization to create excellent early flowering resources of spring Brassica napus L.

Hai-Dong LIU, Yun-Long PAN, De-Zhi DU

表1 SNP和Indel引物序列信息

Table 1 SNP and Indel primers sequence information

引物 Primer 位置 Position (bp) 突变、插入/缺失 Mutate, insert/miss 上游引物序列 Forward primer (5°-3°) 下游引物序列 Reverse primer (5°-3°) SNP6 1636463 G/A CCAGACCCAACATTACCC TCCGGTGTTCTGCTTATT SNP11 2777872 G/A GAAAGCTGCTGGGTTTCT TATTATGCCATGTCATCTAC SNP12 2833887 A/G CTCGGACGAACAGAACCC TCAGCTCATGGAGGAACA SNP14 3147552 T/C TTGAAGTCGTGCGTCGGT AGCAGAGTAGAGGAAGCTGGTA SNP20 3674261 T/C GAGACAAATCTCAGCAGT CAAGATGACGAATCCAAT SNP21 3706251 T/C GTTTATGTCTGACCTTGTT GTGGAACTTGATGGATTT InDel 53 5405790 CA/C ATAGACGTAGAGGCTGAGCA AATCTCGTCACCATCAAGAG InDel 55 5409462 GAATAACATAAAACTT/G GGTGTTCATCTCTGAAGACAA TTGTGATTTGTGAATTGATGA InDel 60 5527563 GTCGC/G CATGCTTCATCTCTTCAACA GTCAACCAGCGAGTAGAAAC