甘蔗PsbS亚基应答甘蔗花叶病毒侵染及其与6K2蛋白的互作研究

张海, 刘淑娴, 杨宗桃, 王彤, 程光远, 商贺阳, 徐景升

Sugarcane PsbS subunit response to Sugarcane mosaic virus infection and its interaction with 6K2 protein

Hai ZHANG, Shu-Xian LIU, Zong-Tao YANG, Tong WANG, Guang-Yuan CHENG, He-Yang SHANG, Jing-Sheng XU

表1 本研究使用的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 策略 Strategy 221-ScPsbS-F GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGCAGTCCATGCT 双分子荧光互补载体构建 221-ScPsbS-R GGGGACCACTTTGTACAAGAAAGCTGGGTCCTACTCTTCGTCCTCGC Vector generation for BiFC ScPsbS-qF CTCCATCATCGGCGAGATCATCAC 定量PCR ScPsbS-qR GGCGGCGACGAAGAAGAAGAG Real-time-qPCR GAPDH-F CACGGCCACTGGAAGCA 内参基因 GAPDH-R eEF-1α-F eEF-1α-R TCCTCAG GGTTCCTGATGCC TTTCACACTTGGAGTGAAGCAGAT GACTTCCTTCACAATCTCATCATAA Reference gene 内参基因 Reference gene SCMV-CP-F TACAGAGAGACACACAGCTG SCMV检测 SCMV-CP-R ACGCTACACCAGAAGACACT Detection of SCMV