甘蔗PsbS亚基应答甘蔗花叶病毒侵染及其与6K2蛋白的互作研究

张海, 刘淑娴, 杨宗桃, 王彤, 程光远, 商贺阳, 徐景升

Sugarcane PsbS subunit response to Sugarcane mosaic virus infection and its interaction with 6K2 protein

Hai ZHANG, Shu-Xian LIU, Zong-Tao YANG, Tong WANG, Guang-Yuan CHENG, He-Yang SHANG, Jing-Sheng XU

图6 BiFC检测ScPsbS与SCMV-6K2的互作 A: YC融合于ScPsbS的C末端, YN融合于SCMV-6K2的N末端; B: YN融合于ScPsbS的N末端, YC融合于SCMV-6K2的C末端。将YN-6K2和ScPsbS-YC(A), YN-ScPsbS和6K2-YC(B)分别共注射到本氏烟叶片中进行瞬时表达, 48 h后激光共聚焦观察。标尺= 25 μm。

Fig. 6 BiFC assays for protein-protein interaction between ScPsbS and SCMV-6K2 A: the C-terminal half of YFP was fused to the C-terminal of ScPsbS to generate ScPsbS-YC, while the N-terminal half of YFP was fused to the N-terminla of SCMV-6K2 to generate YN-6K2; B: the N-terminal half of YFP was fused to the N-terminal of ScPsbS to generate YN- ScPsbS, while the C-terminal half of YFP was fused to the C-terminal of SCMV-6K2 to generate 6K2-YC. Plasmids combination of YN-6K2 plus ScPsbS-YC (A), YN-ScPsbS plus SCMV-6K2-YC (B) were individually co-injected into N. benthamiana leaves for transient expression. The fluorescent signal was monitored by confocal microscopy at 48 h after infiltration; bar = 25 μm.